Evaluation Of The Antidiabetic Effects Of Water And Methanolic Extracts Of Avocado (Persea Americana) Seed On Alloxan Induced Diabetic Rats
Abstract
The high frequence, complications and cost of conventional medicines in the operation diabetes needed the hunt for indispensable treatment. As a result, this study seeks to estimate the composition,anti-diabetic implicit toxin and towel-defensive goods of both the water and methanolic excerpts of Persea americana( avocado pear) seed on alloxan- convinced diabetic albino rats were delved . This study was conceived and designed grounded on information on the original use of the seed in diabetes treatment. Proximate andanti-nutritional ingredients of the seed were determined and 100g of the sample was uprooted with 1000 ml of both water and methanol using the maceration system. The excerpts were faded to blankness using a rotary evaporator and stored at 4oC until use. The goods of different boluses( 200mg/kg.b.wt., 300/kg.b.wt.) of both water and methanolic excerpts ofP. americana seed on alloxan- convinced diabetic albino rats were compared with those of a reference medicine, insulin. The glucose position and weight of the rats were measured daily for 21 days. The liver function tests and the histopathologies of the liver, and feathers, were delved . Results of the proximate disquisition shows that the seed is rich in carbohydrate(49.03 ±0.02 g/ 100g), lipid(17.90 ±0.14 g/ 100g), protein(15.55 ±0.36 g/ 100g) humidity(15.10 ±0.14 g/ 100g) and ash(2.26 ±0.23 g/ 100g). Anti nutritive factors similar as total oxalate(14.98 ±0.03 mg/ 100g), tannin(6.98 ±0.04 mg/ 100g) and phytic acid(3.18 ±0.16 mg/ 1 00g). Results also showed that both the water and methanolic excerpts displayed significantanti-diabetic goods on the experimental rats. still, the methanolic excerpts showed a better anti diabetic effect than the water excerpts. The excerpts showed no significant goods on the liver function parameters( bilirubin, conjugate bilirubin, AST, ALP and ALT) compared with the normal control but rather reversed the histopathological damage that passed in alloxan- convinced albino diabetic rats. In conclusion, the present study provides a pharmacological base for the traditional use ofP. americana seeds excerpts in the operation of Diabetes mellitus. It seemsP. americana seed contains substantial quantum of nutrients that could warrant its application in beast feed or food. still, farther studies are needed to indentify the active component responsible for theanti-diabetic parcels of the seed excerpt.
Chapter One
Preface
Background
During the once decade, the traditional systems have gained significance in the field of drug. The World Health Organization estimates that 4 billion people, 80 of the world population( WHO, 2002), presently use herbal drug for some aspect of primary health care( Orisataoki and Oguntibeju, 2010). Eighty percent( 80) of African populations use some form of traditional herbal drug( WHO, 2002.) and the worldwide periodic request for these products approachesUS$ 60 billion( Willcox and Bodeker, 2004). Herbal drug is a major element in all indigenous people’s traditional drug. Medicinal shops have continued to attract attention in global hunt for effective styles of using shops corridor(e.g. seeds, leaves, stems, roots, barks etc) for the treatment of numerous conditions affecting humans( Sofowora, 2008). This is as a result of the nonstop need for less precious means of complaint control.
Medicinal shops are shops which can be used for remedial purposes or which are precursors for the conflation of useful medicines( Sofowora, 2008). numerous important medicines used in healthcare moment are directly deduced from shops due to its bioactive ingredients similar as; alkaloids, tannins, steroids, etc. exemplifications include L- Dopa deduced fromMucuna spp used foranti-parkinsonism, another is Caffeine, a CNS goad deduced from Camellia sinensis and Quinine from Cinchona ledgeriana and used for Antimalarial, antipyretic.
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