THE EFFECT OF Cisampelous Owarensis BENZENE FRACTION ON GLUCOSE ABSORPTION ACROSS THE INTESTINAL EPITHELIUM OF WISTER RATS ON ALOXAN INDUCED DIABETES

THE EFFECT OF Cisampelous Owarensis BENZENE FRACTION ON GLUCOSE ABSORPTION ACROSS THE INTESTINAL EPITHELIUM OF WISTER RATS ON ALOXAN INDUCED DIABETES

ABSTRACT

Diabetes is a global public health concern that threatens all nations’ economies, particularly those in underdeveloped countries. It is characterized by hyperglycemia and hyperlipidaemia, which can lead to serious morbidities. The purpose of this study was to see how Cissampelos owanrensis affected glucose absorption in alloxan-induced diabetic male Wistar rats. Twenty (20) adult Wistar rats were split into four (4) groups of five (n=5) rats each. Groups 1 and 2 served as controls, whereas groups 3 and 4 were given an intraperitoneal injection of alloxan (40mg/kg) to induce diabetes. Cissampelos owanrensis 300mg/kg was given to the normoglycemic groups (1 and 2) and the alloxan-induced diabetic groups (3 and 4). The animals’ fasting glucose levels were measured at 1 hour, 3 hours, and 4 hours. The rats were sacrificed by cervical dislocation at the end of the experiment, and blood samples were centrifuged to extract serum for biochemical analysis. The duodenum, jejunum, and ileum were all removed for biochemical analysis. The acquired data were analyzed using the SPSS statistical software and represented as meanSEM. The results reveal that diabetic rats’ fasting blood glucose levels were dramatically reduced after receiving 300mg/kg of the plant extract (502.335.57 after 1 hour, 427.337.67 after 3 hours, and 298.3310.91 after 4 hours). Finally, benzene fractions of Cissampelos owanrensis reduced glucose absorption across the intestinal epithelium significantly.